This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin induced diabetes that were chronically exposed to f. Again, this insulinopenia syndrome, called streptozotocin diabetes, is caused by the specific necrosis of the pancreatic beta cells and streptozotocin has been the agent of choice for the induction of diabetes mellitus in animals ever since 3, 16. This difference can be significant, with little or no response in female mice and severe hyperglycemia present in. Rats were treated with aqueous leaf extract 100 mgkg or agnps 10 mgkg for 28 days. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. Streptozotocin stz is a naturallyoccurring small molecule mw265 that is specifically toxic to pancreatic. The functional insufficiency of the recovery in this period 712 months after streptozotocin application may be uncovered by. It is used in medicine for treating certain cancers of the islets of langerhans and used in medical research to produce an animal model for hyperglycemia and alzheimers in a large dose, as well as. Stztreated rats or mice represent an induced diabetes model, which is especially useful for studying agents that treat type i diabetes such as insulin replacement or adjunctive therapies. Manganese was given by ip injection of 15 mg manganese chloridekgday in the saline solution for a period indicated in each group.
Streptozotocininduced type 1 diabetes in rodents as a. Murugesan 2 1 department of biochemistry, kanchi shri krishna college of arts and science, kilambi. As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute. Streptozotocininduced diabetes in rats is associated with impaired metabolic availability of vitamin a retinol volume 75 issue 4 p.
Administration of high doses of fluoride f can alter glucose homeostasis and lead to insulin resistance ir. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. Hyperglycemia enhances the production of reactive oxygen species, leading. Streptozotocininduced diabetic ratsmice stz model advantages. Stz induced diabetes are commonly performed in male wistar kyoto rats. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. After treatment, in the pancreas no insulitis was observed. Antidiabetic properties and mechanism of action of orthosiphon stamineus enth bioactive subfraction in streptozotocin induced diabetic rats. Stz is well known to cause pancreatic bcell damage, whereas na is administered to rats to partially protect insulinsecreting cells against stz.
Streptozotocininduced type 1 diabetes in rodents as a model. Diabetes is associated with declining sexual function in women. Proteomic analysis of gastrocnemius muscle in rats with. Streptozotocininduced diabetic models in mice and rats. In the present study streptozotocin dose was as 50mgkg body weight in study of zafar16, m et al used 50mgkg bw stz dose, in study of mozaffari et al23 used 90 mgkg body weight. The functional insufficiency of the recovery in this period 712 months after streptozotocin application may be uncovered by the glucose tolerance test.
The responses are accompanied by corresponding inverse changes in. Evaluation of antidiabetic activity of biologically. Multiple low dose streptozotocininduced diabetes in the rat causes acute upregulation of plasma osteopontin opn. Streptozotocininduced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic. Diabetes was induced in 8 week old male wistar rats n96 using a single rapid injection of streptozotocin stz. Effects of streptozotocin diabetes in the rat on blood. Detailed in this unit are protocols for producing stz. Urinary metabolomics study on the protective role of. Streptozotocininduced diabetes in laboratory animals presents an experimental model whose value is in the elucidation of causal relationships related to human diabetes mellitus. The mechanism of alloxan and streptozotocin action in b cells.
The mechanisms of alloxan and streptozotocininduced. Antihyperglycemic effect of an extract of myrtus communis in streptozotocin induced diabetes in mice. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. Guidelines on use of streptozotocin in rodents ulam. Improving the reliability and utility of streptozotocin. In this study, a 1h nmr based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of os in dm using streptozotocin stz induced experimental model in rats. Four different solvent extracts of os, namely aqueous, ethanolic, 50% aqueous ethanolic. However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. Effect of streptozotocininduced diabetes on pancreatic. Can anybody tell me a drugcompound to be used as method control. Three days after degeneration of beta cells, diabetes was induced in all animals. Other models of diabetes include genetic models and the use of a prolonged highfat diet regimen. Diabetes was produced in fiveday pregnant sprague dawley rats by intravenous injection of 40 mg.
Murugesan2 1department of biochemistry, kanchi shri krishna college of arts and science, kilambi. This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocininduced diabetes that were chronically exposed to f. Usually at week 3, most stzinjected mice develop severe diabetes with blood glucose. Sonnenblick, and james scheuer summary in order to determine whether diabetic cardiomyopathy in. Stz model, streptozotocininduced diabetes model melior. Changes in oxidative stress and antioxidant enzyme activities. I have been working on stz induced diabetes type i model in rats. For inducing diabetes, a single iv injection of 60 mg streptozotocin kg in a physiological saline solution was given at the time indicated below. Effects of streptozotocin induced diabetes on neuronal sigma receptors in the rat brain. The streptozotocin stz induced diabetic model is widely used. Please visit guidelines on use of streptozotocin in rodents to locate the document you need. Oral administration of dahi containing probiotic lactobacillus acidophilus and lactobacillus casei delayed the progression of streptozotocin induced diabetes in rats volume 75 issue 2 hariom yadav, shalini jain, pushpalata ravindra sinha. Multiple low dose streptozotocininduced diabetes in the.
The effect of streptozotocininduced diabetes in rats on. Firstly, it closely resembles human type 1 diabetes with chronic pancreatic islet inflammation, insulitis, and insulin. Multiple low dose streptozotocin induced diabetes in the rat causes acute upregulation of plasma osteopontin opn. Male mice and rats tend to be more susceptible to stzinduced diabetes 10, 15, 16, 2225.
The stz model provides a good, simple screening tool to test compounds and to further understand the complex development of neuropathic pain. The advantages of nongenetic animal models of diabetes compared with the genetically. Streptozotocin is a glucosamine nitrosourea compound. This difference can be significant, with little or no response in female mice and severe hyperglycemia present in male mice receiving identical doses. Novel insight into streptozotocininduced diabetic rats from. The autoimmune disorder is characterized by the destruction of insulinproducing beta cells in the pancreas by the patients immune system. Protective role of opn in the islets osteopontin opn is a secrete osteopontin opn is a secreted acidic phosphoprotein that binds to a cell surface integrinbinding motif and is involved in many inflammatory and immunemodulating disorders. In conclusion, the streptozotocininduced diabetes mellitus in rats was stable for 6 months, afterward, however, a significant recovery developed in. Stz induced diabetes in rats is a valuable tool for diabetes research which has been used by many investigators. Sep 14, 2017 experimental model of stz induced diabetes. Depending on the animal strain, dose, route of drug administration, and the life period in which stz is administered in rats, severe diabetes blood glucose superior to 200300 mgdl 7177 or mild diabetes glycemia between 120 and 200300 mgdl are generated 68, 7881. Rodents also show a substantial gender difference in stz sensitivity.
Type 1 diabetes t1d is a pathological conditioncharacterized by the loss of insulinproducing. Treatment with streptozotocin increases blood glucose levels in mice by itself before the septic challenge figure 1a. With the use of pinhole collimators and the advance in data processing, gated spect was recently adapted to image the rat heart 14. For example, in rats with streptozotocininduced diabetes, transfection of human tissue kallikrein gene has been reported to prevent diabetic nephropathy riad et al.
Intravenous injection of 60mgkg dose of streptozotocin in adult wistar rats, makes pancreas swell. Streptozotocin stz is toxic to pancreatic islet insulinproducing bcells, thus inducing diabetes. Chronic hyperglycemia and the corresponding glucotoxicity are the main pathogenic. Effect of streptozotocininduced diabetes on left ventricular. Diabetes was induced with streptozotocin, the standard and most common method for experimental diabetes 35, 36,41. Effect of streptozotocin on glucose levels in albino. This study investigated the role of heme oxygenase ho1 in the cardiac tissue injury of acute ischemiareperfusion ir in diabetic streptozotocin stzinduced hyperglycemic rats. The document you are looking for is no longer available at this address. Multiple administrations of lowdose stz are employed to produce diabetic mice. Following treatment, rats were killed for biochemical and histopathological analysis of kidney and liver samples.
In control and streptozotocintreated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. Type 1 diabetes mellitus was induced in rats by the intraperitoneal injection of freshly prepared streptozotocin stz at a dose of 45 mgkg dissolved in 0. Abstractadministration of high doses of fluoride f can alter glucose homeostasis and lead to insulin resistance ir. Ihave used tolbutamide, based on several papers, but it didnt even work slightly. From the above results it is shown that the treatment. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm. Streptozotocin stzinduced diabetic model creative biolabs. Nonfasting blood sugar of the streptozotocintreated. Collectively, our data indicate that bge, in part by suppressing cytokineinduced apoptotic signaling, protects. Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself.
Multiple low dose streptozotocininduced diabetes in the rat. In this study, diabetes was induced by a single injection of streptozotocin stz, 45 mgkg, i. The most widely used stzinduced diabetic model is elicited in mice. Streptozotocin induced diabetic rat model introduction type 1 diabetes is an autoimmune disorder that affects nearly 347 million people worldwide. This model can be established in genetically modified rodents for investigating the role of molecular mechanisms and genetic susceptibility in the development of diabetic nephropathy. Streptozotocininduced diabetic models in mice and rats article in current protocols in pharmacology september 2015 doi. Stz diabetic rats were treated with oral doses of mebs 200 and 400 mgkg for 8 weeks.
Nov 28, 2012 diabetes was induced in rats by intraperitoneal administration of 60 mgkg of streptozotocin stz. Wistar rat, male, 250g body weight streptozotocin stz induction protocol. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin.
Effects of streptozotocininduced diabetes on neuronal. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis hyperglycemia in some animal species. May 30, 2006 diabetes is associated with declining sexual function in women. Antidiabetic properties and mechanism of action of orthosiphon stamineus enth bioactive subfraction in streptozotocininduced diabetic rats.
Streptozotocininduced diabetic neuropathy in mice and rats. In control and streptozotocin treated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. To improve the reliability and utility of this model, we characterize the age dependence of stz toxicity and introduce novel endpoints to assess diabetic complications and reveal possible mechanisms for diabetic development. Similarly, in experimental models of diabetes, overexpression of ace and hence, lower levels of bradykinin, are associated with worsening of diabetic nephropathy riad et. Streptozotocininduced diabetes in the rat is associated with. Ultrastructural analysis of in vivo hypoglycemiant effect of two polyoxometalates in rats with streptozotocininduced diabetes volume 21 supplement stefana balici, modeste wankeunya, dan rusu, gheorghe z. Full text streptozotocininduced type 1 diabetes in. Full text streptozotocininduced type 1 diabetes in rodents.
Streptozotocininduced diabetes in the rat is associated. Diabetes was induced by stz injection into male, 6 to 23 weeks old, spraguedawley rats. Pancreases of the mothers and their offsprings were extracted by acidalcohol and the insulin measured by immunoassay using rat insulin as a standard. Three days after the stz injection, the blood was withdrawn from the tail vein, and the glucose level. The effects of 1 hemin, an inducer of ho expression and activity, and 2 zinc protoporphyrin ix znppix, an inhibitor of ho activity, have also been investigated on the tissue injury by ir and some. Streptozotocininduced diabetic rat model background strain. Streptozotocin stz is a widely used chemical for the induction of experimental diabetes in rodents. The effect of streptozotocininduced diabetes in rats on cardiac contractile proteins ashwani malhotra, somsong penpargkul, frederick s. In these models, male wistar rats at 8 weeks of age 200300 g are selected from animal supply facility of sgpgims lucknow, and house in a 12h light, 12h dark cycle environment, temperature 25c, with standard rat diet and water in metabolic cages for one week prior to. To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. When treated with stz, the animals developed severe hyperglycaemia, which differed substantially from the levels of the control rats.
Streptozotocininduced experimental diabetes in male wistar rats. Oct 15, 2007 different imaging modalities have been used to asses the onset and time course of lv function abnormalities in small animals with streptozotocin stz induced diabetes, especially in rats 7. Comparison of streptozotocin and alloxaninduced diabetes in. Administration of both streptozotocin stz and nicotinamide na has been proposed to induce experimental diabetes in the rat. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. Type 1 diabetes t1d is a pathological condition characterized by the loss of insulinproducing. However, stz diabetes is multifactorial and depends on various practical aspects such as stability of stz, animal species, strain, gender, age, route of administration and dosage. The present study was intended to provide an insight into the specific effects of progesterone, the other female sex hormone, in the diabetic renal complications. Antidiabetic activity of different extracts of myrtus. Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. Hypoglycemic effect of carica papaya leaves in streptozotocin.
Streptozotocin use in mice and rats version 1 date of publication. The mechanisms of alloxan and streptozotocininduced diabetes. Further, in vivo antidiabetic activity was assessed in streptozotocin. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin installed and enabled in your browser. Streptozotocinnicotinamideinduced diabetes in the rat. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. The success rate of inducing diabetes defined as a blood glucose concentration. Male wistar rats 60 days old were randomly distributed into two groups of 18 animals. In rats, streptozotocin stz induces diabetes when used at doses ranging from 45 to 70 mgkg rakieten et al. For induction of experimental diabetes in male adult rats weighted 250300 grams 7590 days, 60mgkg of streptozotocin was injected intravenously. Skeletal system of rats with streptozotocininduced diabetes. Antihyperglycemic effect of an extract of myrtus communis in streptozotocininduced diabetes in mice. In the diabetic rat trial, diabetes was induced in overnight fasted rats by intraperitoneal injection of streptozotocin dissolved in 0.
153 1182 151 69 1515 1529 1008 1001 724 380 1167 1308 107 186 1510 1466 37 324 1335 1369 987 1000 334 902 1563 1309 1559 1176 375 1448 209 905 77 504 599 1044 979 431 708 1362 1406 128 309 456 831 912 541